Responsiveness to microbe-associated molecular patterns in Brachypodium distachyon
A. TAKAHASHI (1), Y. Kouzai (1), M. Kimura (1), M. Watanabe (1), H. Matsui (1), M. Yamamoto (1), Y. Ichinose (1), K. Toyoda (1), Y. Noutoshi (1) (1) Okayama University, Japan

Brachypodium distachyon (purple false brome) is a grass plant of the Pooideae subfamily, which includes economically important crops such as wheat, barley, rye and oats. Owing to its small stature, short lifecycle, self-fertility and small diploid genome (272Mbp), Brachypodium can be an experimental model plant for studies of monocotyledonous plants. A whole-genome sequence, a database of full-length cDNA (FLcDNA) and collections of mutants and accessions are available. Since various pathogens threatening world crop cultivation such as Fusarium graminearum and Magnaporthe oryzae can infect to Brachypodium, it may also be a useful platform to investigate both pathogen virulence and plant immune response at the molecular level. Here we analysed responsiveness of Brachypodium to various microbe-associated molecular patterns (MAMPs) such as chitin, lipopolysaccharide, peptidoglycan, flg22 peptide and elf18 peptide. Production of reactive oxygen species (ROS) to various MAMPs was favorably measured using leaf discs and luminol but not L-012. ROS productions were detected to all the tested MAMPs from 0.5 to 3 h. The intensity peaks of ROS were around 2 h except for flg22 with 1 h. We then successfully identified 8 marker genes for the monitoring of MAMP response based on the sequence similarity to chitin responsive genes in rice. Their expressions in leaf discs were detected 6 h after the treatments of each MAMP.

Abstract Number: P17-621
Session Type: Poster