Bioactivities of Trichoderma chrysophanol
S. LIU (1), C. Liao (2), M. Hsieh (1), C. Lo (3), H. Yang (4), K. Lin (2), K. Peng (2) (1) Department of Molecular Biotechnology, Da-Yeh University, Taiwan; (2) Department of Life Science, National Dong Hwa University, Taiwan; (3) Department of Biotechnology, National Formosa University, Taiwan; (4) Department of Research, Buddhist Tzu Chi General Hospital, Taiwan

Trichoderma spp. effectively stimulates plant growth and enhances disease resistance against various phytopathogens. In this study, we elucidated the effect of chrysophanol, a secondary metabolite of Trichoderma harzianum, on the growth and disease resistance of cabbage (Brassica oleracea var. capitata) against Botrytis cinerea in a 3-way interaction. Our results revealed that chrysophanol assists cabbage growth and promotes resistance against Botrytis in a similar manner to Trichoderma. The results of proteomic analyses indicated that proteins related to photosynthesis were highly expressed in the leaves of Trichoderma- and chrysophanol-treated cabbages. The transcription levels of photosynthesis-related genes increased dramatically in response to chrysophanol treatment, and the expression profiles were similar to that of Trichoderma treatment. The sucrose transport-related genes increased significantly in chrysophanol-treated cabbage as well. The transcription levels of b-1,3-glucanase, pathogenesis-related protein 1, glutathione S-transferase, and deoxycytidine deaminase decreased in both Trichoderma- and chrysophanol-treated cabbages, whereas the transcription levels of ascorbate peroxidase increased in both treatments. The results of this study revealed that chrysophanol functions are similar to those of Trichoderma, & strongly indicating that chrysophanol is crucial in stimulating plant growth and CO2 fixation and in eliciting host defense responses during the colonization of Trichoderma at the rhizosphere.

Abstract Number: P17-561
Session Type: Poster