Involvement of a putative SlSOBIR1-interacting receptor-like protein in plant response to elicitins
R. LIOU (1), W. Shih (1), C. Huang (1), C. Wang (2), K. Peng (1) (1) Dept. Plant Pathol. & Microbiol., National Taiwan University, Taiwan; (2) Institute of Plant & Microbial Biology, Academia Sinica, Taiwan

In response to infection by Phytophthora pathogens, plants elicit the first layer of innate immunity, pattern-triggered immunity (PTI). To date, a variety of pathogen-associated molecular patterns have been identified in Phytophthora spp., including elicitins, which induce defense responses and cell death in a limited range of dicot plants. In Nicotiana benthamiana, INF1 (an elicitin of Phytophthora infestans)-induced cell death requires tobacco homolog of BRI1-ASSOCIATED KINASE1 (BAK1) and NbLRK1, a lectin-like receptor kinase. Moreover, tomato homolog of SUPPRESOR OF BIR1 (SlSOBIR1) plays an essential role in the defense signaling triggered by ParA1, the elicitin from Phytophthora parasitica, and in plant basal defense against this pathogen as shown in our recent study. To understand how SlSOBIR1 activates signaling upon ParA1 perception, we performed coimmunoprecipitation and mass spectrometry after treatment of N. benthamiana with ParA1 and identified 157 putative SlSOBIR1-associated proteins. In this study, we provide evidences that one of these proteins, named Nicotiana benthamiana RECEPTOR-LIKE PROTEIN 1 (NbRLP1), is involved in elicitin signaling in plants. The expression of NbRLP1 is induced when plants are infected by P. parasitica. Transient expression by agroinfiltration indicates that NbRLP1-GFP is localized predominately in the plant plasma membrane. However, it is translocated from the plasma membrane to endosomal compartments upon ParA1 treatment. Overexpressing NbRLP1 on tobacco leaves accelerates the appearance of necrosis caused by ParA1. These results suggest an important role of NbRLP1 in plant response toward elicitins.

Abstract Number: P17-609
Session Type: Poster