Identification of infection-specific proteins produced in Japanese birch plantlet No.8 infected with Inonotus obliquus strain IO-U1   
S. YOKOTA (1), S. Iizuka (2), J. Ohshima (2), F. Ishiguri (2) (1) Utsunomiya University, Japan; (2) Utsunomiya University, Japan

We have been investigating the responsive reactions of Japanese birch (Betula platyphylla var. japonica) plantlets infected with a canker-rot fungus Inonotus obliquus strain IO-U1 by using proteomics. Our previous study identified two fungal infection-specific proteins, HSP70 and HSP60 using peptide mass fingerprinting method. However, many specific proteins have remained unidentified. Hence, in this study, we aimed to identify remaining unidentified specific proteins using peptide sequence tag (PST) method. Fungal mycelium was inoculated to V-shaped cut at the 3rd internode from the apex of the birch plantlet (T). As controls, non-treated (C1) and V-shaped cut plantlets (C2) were also prepared. After 2 days from the treatments, each plantlet was collected, and proteins were extracted with buffer and subjected to two-dimensional electrophoresis. The gels were stained with Coomassie brilliant blue (CBB), and the specific protein spots were detected and subjected to in-gel-digestion with trypsin. The obtained peptides were analyzed by LC/MS/MS, and the proteins were identified by data base search. Total 828, 827, and 841 proteins were detected by CBB staining in T, C1, and C2 plantlets, respectively. Twelve proteins were detected as fungal infection-specific proteins in T plantlet. These proteins were analyzed and identified by PST method. All twelve proteins were identified and categorized into the following six groups: energy production, protein synthesis, protein degradation, protein restoration, stress response, and metabolism. We consider that these identified proteins are significantly involved in the responsive mechanisms of birch plantlet against the fungal infection.    

Abstract Number: P18-697
Session Type: Poster