Genetic diversity of Rhizobia that nodulate Onobrychis Esparseta from the arid eastern area of Central Asia
B. UMAROV (1), Z. Shakirov (2), M. Sagdieva. (2) (1) Institute of Microbiology AS RUz, 7b A.Kadyri str. Tashkent 100128, Uzbekistan,, Uzbekistan; (2) Institute of Microbiology AS RUz,, Uzbekistan

Nitrogen Symbiotic fixation in the arid regions depends on the two partners of symbiosis: legume - rhizobium and their aptitude to adapt to prevailing climatic and soil constraints. We showed that Onobrychis esparseta is a salt tolerant legume species, since it is not affected by the salt concentrations. This plant represents a very good candidate to combat desertification. We thus isolated 50 bacterial strains from the root nodules of Onobrychis esparseta, cultivated in different soils of the Central Asia. We found, that 5 strains tolerate NaCl concentrations higher than 30 g/l (w/v). All the strains re-nodulated their originals host. They all possess nodC gene. Was cloned nodC gene by using PCR with specific primers. The assessment of metabolic and physiologic properties of these isolate showed a high phenotypic diversity. This is an advantage to the plant since it can be always nodulated by different type of rhizobia even if the soil or climate conditions have to change. The RFLP of the 16S rDNA using MspI, HaeIII and CfoI enzymes confirmed the diversity of the strains that nodulate this species in the same soil. We thus obtained three different profiles. The diversity of the strains was also assessed by the exploration of the bacterial chromosome carried out by rep PCR using BOX and ERIC primers.The Results obtained showed a high diversity of the strains.

Abstract Number: P2-54
Session Type: Poster