Sequence identification of a novel begomovirus affecting bean crops in southwestern Colombia
M. CARVAJAL-YEPES (1), L. Zambrano (1), J. Bueno (1), C. Olaya (2), W. Cuellar (3) (1) International center for tropical agriculture, Colombia; (2) Washington State University, U.S.A.; (3) International center for tropical agriculture, Colombia

Geminiviruses belong to a fast growing family of plant pathogens. In bean crops, a series of begomoviruses have been reported to affect productivity in Latin America. Bean leaf crumple virus (BLCrV) was first reported in 2002 affecting green beans in the department of Valle del Cauca, Colombia. Diseased plants were described to show severe leaf deformation with mild variegation (incidence of 80%). Since then, in the department of Valle del Cauca, there has been a drastic decrease (60-70%) of the cultivated area of green beans due to recurrent and sporadic outbreaks of begomovirus-associated symptoms. We identified the sequence of the begomovirus species responsible for recent outbreaks of the disease in Colombia and performed phylogenetic analysis for its proper classification. Bean samples collected between 2008 and 2015 in Valle del Cauca were tested for Geminivirus by PCR or for circular DNAs by rolling circle amplification (RCA). PCR tests confirmed the presence of Geminiviruses and RCA allowed to obtain the whole viral genome. Sequence analysis of Rep (Segment A) encoding region showed a 84-97% identity with Euphorbia mosaic Venezuela virus and Tomato severe leaf curl virus and 80-81% identity with BCaMV. Movement protein (MP) (Segment B) has a 93% nt identity with Sida micrantha mosaic virus. The phylogenetic analysis of both segments revealed that all isolated sequences of BLCrV cluster separately from any previously reported begomoviruses infecting beans in Latin America. 

Abstract Number: P11-346
Session Type: Poster