Function of a glutathione-S-transferase in Arabidopsis pre-invasive defence
M. PASTORCZYK (1), M. Pislewska-Bednarek (1), R. Nakano (2), K. Hiruma (2), A. Molina (3), Y. Takano (4), P. Schulze-Lefert (2), P. Bednarek (1) (1) Institute of Bioorganic Chemistry, Poland; (2) Max Planck Institute for Plant Breeding Research, Germany; (3) Centre for Plant Biotechnology and Genomics, Spain; (4) Graduate School of Life and Environmental Sciences, Jordan

In the model plant Arabidopsis the pre-invasive resistance towards a broad range of pathogens is mediated by products of indole glucosinolate (IG) metabolism. The respective defence mechanism involves CYP81F2 P450 monooxygenase, PEN2 myrosinase and the plasma membrane localized PEN3 ABC transporter that delivers end products of the CYP81F2/PEN2-mediated IG metabolism into the apoplast to terminate invasive pathogen growth. Our former analysis suggested a key function of glutathione in the PEN2/PEN3 immune pathway. In this study we investigated the details of the mechanisms underlying glutathione function in Arabidopsis pre-invasive resistance. Gene co-expression analysis indicted candidate glutathione-S-transferases (GSTs) that could be involved in the pathogen triggered IG metabolism. Metabolomic analysis of the corresponding mutant lines indicated that GSTU13 is crucial for the formation of the end products of this pathway. Together with our earlier results, this allowed us to propose precise biosynthetic function of GSTU13 in IG metabolism. Contribution of GSTU13 to the pre-invasive resistance was further confirmed with compromised resistance of gstu13 mutant plants towards selected biotrophic, hemibiotrophic and necrotrophic pathogens. Collectively our data indicate that GSTU13 constitutes a crucial component of the PEN/PEN3 pathway that mediates pre-invasive resistance towards a broad spectrum of fungal pathogens

Abstract Number: P15-431
Session Type: Poster