Genome-based cloning of Pvr4 conferring multiple potyvirus resistance from Capsicum annuum ‘CM334’
S. KIM (1), J. Lee (2), D. Choi (2) (1) Seoul National University, South Korea; (2) Seoul National University, South Korea

Pvr4 is a resistance gene conferring broad dominant resistance against Pepper mottle virus (PepMoV), Pepper severe mosaic virus (PepSMV) and all isolates of Potato virus Y (PVY). Capsicum annuum landrace ‘CM334’ is known to have Pvr4, but it has not been cloned and the mechanism of resistance is unknown. To perform genome-guided cloning of Pvr4, based on synteny between tomato and pepper genomes, a high-density genetic map including thirty-two co-dominant markers was developed in flanking region of TG420. Three CAPS markers co-segregated with Pvr4 in two populations derived from crosses between C. annuum ‘CM334’ and ‘ECW’ or ‘Jupiter’, were developed. Flanking region of Pvr4 was delimited as 175kb containing eight Pvr4 candidates encoding putative coiled-coil (CC) nucleotide-binding leucine rich repeat (NB-LRR). To identify Pvr4, in planta co-expression of the eight Pvr4 candidates and PepMoV-NIb were transiently co-expressed in susceptible pepper (C. annuum ‘Jupiter’) and Nicotiana benthamiana leaves. One of these candidates, designated the Pvr4 gene, induced hypersensitive response (HR) by co-expression with PepMoV-NIb. Furthermore, over-expression of Pvr4 significantly suppressed replication of PepMoV, PepSMV and PVY in N. benthamiana. Pvr4 consists of seven exons and encodes a putative CC-NB-LRR type protein with 1746 amino acids. The identified Pvr4 gene may provide a novel source of resistance for breeding potyvirus resistant crops including Solanaceae plants.

Abstract Number: P17-545
Session Type: Poster