Complete genome sequence and pathogenomic analysis of a Chinese strain GX01 of Xanthomonas oryzae pv. oryzicola
Y. HE (1), X. Niu (2), K. Li (2), Z. Wei (2), S. Carpenter (3), W. Jiang (2), Y. Yu (2), L. Wang (4), S. Huang (2), A. Bogdanove (4), J. Tang (2) (1) College of Life Science and Technology, Guangxi University, China; (2) College of Life Science and Technology, Guangxi University, China; (3) Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, China; (4) Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, U.S.A.

Xanthomonas oryzae pv. oryzicola (Xoc) causes rice bacterial leaf streak, an increasingly serious disease worldwide. The molecular basis of Xoc pathogenicity is incompletey understood, and no resistance genes in rice have been reported. Xoc strain GX01, isolated in the south of China, was completely sequenced using PacBio Single-Molecule Real-Time (SMRT) technology and verified with 454 and Illumina sequences. The genome consists of a circular 4,793,207-bp chromsome and a circular 53,206-bp plasmid with 64.03% and 61.25% GC contents, respectively. 4,516 and 64 protein coding sequences (CDSs) were annotated respectively in the chromsome and plasmid. The genome contains 27 tal (transcriptional activator-like effector) genes, including one truncated at the 3’ end and one shortened at both the 3’ and 5’ end. Five, including the virulence factor tal2g, are conserved in all other sequenced Xoc strains, and 19 have orthologs in certain Xoc strains. Three are novel with respect to number of repeats and encoded repeat variable di-residues. Compared to other sequenced Xoc strains, GX01 contains genes encoding a type IV secretion system, a cobalt-zinc-cadmium resistance efflux system?and a Tn3-related mobile insertion cassette, a presumed carrier for pathogenicity-related genes. By our estimate, GX01 has the greatest number (323) and diversity of pathogenicity related and stress adaptation genes of any sequenced Xanthomonas. This complete genome sequence may provide new insights into the molecular arms race between this formidable pathogen and rice.

Abstract Number: P11-360
Session Type: Poster