PLDgamma1 is a negative regulator of MAMP signalling in Arabidopsis thaliana
M. SCHLOEFFEL (1), M. Šemanjski (2), W. Wan (3), B. Macek (2), A. Gust (3) (1) Plant Biochemistry, Center for Plant Molecular Biology, Germany; (2) Proteome Center Tuebingen Interdepartmental Institute for Cell Biology, Germany; (3) Plant Biochemistry, Center for Plant Molecular Biology, Germany

Upon microbial infection plant innate immunity is activated by the recognition of MAMPs (microbe-associated molecular patterns) such as chitin from fungi or the 22 amino acid peptide from bacterial flagellin, flg22. Chitin is recognized by LysM receptor-like kinases (RLKs) like CERK1 and LYK5 whereas flg22 requires the LRR-RLK FLS2 and its co-receptor BAK1. We found a member of the phospholipase D family, PLDγ1, to be involved not in chitin-triggered immune responses but also in flg22 signalling. Generally, the activation of phospholipases leads to the production of important defence signalling molecules, such as oxylipins and jasmonates, as well as the potent second messenger phosphatidic acid (PA), which has been shown to modulate the activity of a variety of proteins involved in defence signalling. Genetic inactivation of PLDγ1, but not its closest homologs PLDγ2 and PLDγ3, resulted in an enhanced production of reactive oxygen species upon elicitation. Moreover, pldγ1 mutants were more resistant towards infection with Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000. PLDγ1 might thus be a negative regulator of MAMP signalling.

Abstract Number: P17-603
Session Type: Poster