Genome-wide transcriptome analysis of a plant growth-promoting phototrophic bacterium, Rhodopseudomonas palustris strain PS3 
C. LIU (1), K. Lo (2) (1) National Taiwan University, Taiwan; (2) Institute of Biotechnology, National Taiwan University,, Taiwan

We isolated a phototrophic bacterium, Rhodopseudomonas palustris strain ??????????????, are that the thPS3 (PS3) from Taiwanese paddy soil. It could not exert beneficial effects on plant growth, but also enhance the efficiency of uptake of the applied fertilizer nutrients in soil cultivation system. While application of the PS3 inoculant in the hydroponic system, the yields of several leafy vegetables were also significantly increased. Furthermore, this treatment resulted in greatly reduction of the nitrate accumulation in vegetables. We determined the whole genome sequence of PS3 and an ineffective R. palustris strain YSC3 (C3) with next-generation sequencing (NGS). The draft genomic sizes of the PS3 and the C3 are 5,318,369 bp and 5,381,463 bp, respectively. The deduced protein-coding genes of PS3 and C3 strains are 5,732 and 5,803, respectively. To elucidate the underlying mechanisms for plant growth promotion by the PS3, we conducted whole-transcriptome analyses. The gene expression profilings of PS3 and C3 under free-living and plant-beneficial conditions in hydroponic system were compared. We have evaluated the gene expressions of those associated with nutrient utilization, mobility, chemotaxis and nitrogen fixation, etc. We expect the findings of this study will give new insights into PGPR, and help improve their effectiveness and stability in agricultural practices.

Abstract Number: P2-36
Session Type: Poster