Dissecting the role of NbRab small GTPase in different plant viruses
C. CHENG (1), P. Hou (1), P. Chen (1) (1) Tzu Chi University, Taiwan

Bamboo mosaic virus (BaMV) is a single-stranded positive strand RNA virus. Several host factors were identified through cDNA-AFLP. Among those, NbRABG3f expression was found to be up-regulated in the BaMV-inoculated N.benthamiana. NbRABG3f small GTPase is a Rab7 homolog protein which involved in the vesicle trafficking within cells. Previous studies revealed that NbRABG3f is likely derived from the Golgi membrane and participates mainly in BaMV replication. However, although NbRABG3f expression was enhanced after Cucumber mosaic virus (CMV) inoculation, the accumulation of NbRABG3f protein was reduced and likely due to degradation by CMV infection. Transient expression of NbRABG3f inhibited CMV coat protein accumulation at beginning. However, CMV dramatically increased and NbRABG3f decreased after 3 dpi. These results suggest that NbRABG3f may negatively regulate CMV replication. 

Abstract Number: P7-161
Session Type: Poster