Quantitative monitoring of pathogen-induced plant hormone dynamics with temporal and single cell resolution
H. GHAREEB (1), S. Laukamm (1), M. Pound (2), O. Tetyuk (3), K. Hanika (4), V. Lipka (3) (1) Department of Plant Cell Biology, Georg August University of Goettingen, Germany; (2) Centre for Plant Integrative Biology, University of Nottingham, United Kingdom; (3) Department of Plant Cell Biology, Georg August University of Goettingen, Germany; (4) University of Wageningen, Netherlands

Plant hormones are important regulators of development and mediate responses to both biotic and abiotic stresses. Pathogen attack is known to alter hormonal homeostasis in the host either leading to activation or suppression of plant innate immunity. Although the roles of some plant hormones, such as salicylic acid, jasmonic acid and ethylene have been extensively studied in plant defense, maintenance of hormonal homeostasis, as well as their fine-tuned crosstalk and antagonistic interactions is still poorly understood, in particular at cellular resolution. To better understand the role of plant hormones during plant-microbe interactions, quantitative tools allowing studies on cellular activity and crosstalk are required. In this regard, we established a platform called “COLORFUL Biosensors” in order to resolve hormone activity networks on a cellular level. “COLORFUL Biosensors” exploits the full spectrum of available fluorescence proteins to generate hormonal reporters that can be genetically transferred to plants. Moreover, we developed a high-throughput bioinformatic tool for sensor quantification. We will report on our recent progress using our platform to resolve spatio-temporal dynamics of hormone responses during plant-pathogen interactions on the single cell level.

Abstract Number: P16-449
Session Type: Poster