Comparative Analysis of Signaling Pathways Triggered by Different Pattern-recognition Receptor-types 
W. WAN (1), M. Zaidem (2), J. Kilian (1), D. Weigel (2), A. Gust (1), T. Nuernberger (1) (1) Center for Plant Molecular Biology and Plant Biochemistry, University of Tuebingen, Germany; (2) Department of Molecular Biology, Max Planck Institute for Developmental Biology, Germany

Plant cell surface receptors sense microbial pathogens by recognizing microbial structures called pathogen or microbe-associated molecular patterns (PAMPs/MAMPs). There are two major types of plant pattern recognition receptors: 1. Leucine-rich repeat receptor proteins (LRR-RP) and leucine-rich repeat receptor kinases (LRR-RK) and 2. Plant receptor proteins and receptor kinases carrying ectopic lysin motifs (LysM-RP and LysM-RK). Although many studies focused on the signal pathways triggered by these receptors individually, the exact overlap and the differences, respectively, between these pathways remain unknown. We use three different PAMPs, flg22, nlp20, chitin (chitohexaose), and their corresponding receptor types, FLS2 (RLK), RLP23 (RLP), CERK1 (LYM-RK) for our comparisons. By systematic analyses of defense responses such as transcriptome changes, ROS burst and accumulated hormones, we found that flg22 triggers faster and stronger early responses, and also causes more extensive transcriptome changes. On the other hand, nlp20-treatment results in high amounts of salicylic acid and camalexin accumulation. Proteins like BIR2 and CPK28, which participate in RLK-related pathway, also play roles in RLP-related pathways as negative regulators of PTI according to our study. We hope to draw a more complete picture of PAMP-triggered immunity and to discover new key components participating in plant immunity.

Abstract Number: P16-478
Session Type: Poster